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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital function in promoting cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already described. Two supplemental splice variants had been described but comprehensive nucleotide sequence facts will not be however out there.
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Blocking peptides are peptides that bind specially on the concentrate on antibody and block antibody binding. These peptide normally has the epitope identified with the antibody.
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This tutorial outlines more possible optimization of mobile and immunoassay parameters to make sure the very best final results are...
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This guideline outlines even further achievable optimization of cellular and immunoassay parameters to be sure the absolute best effects are...
Antibodies bound to the blocking peptide not bind on the epitope to the concentrate on protein. By comparing the staining from your blocked antibody vs . the antibody by yourself, one can see which staining is particular.
Concentrate on Information and facts Cyclin E belongs towards the extremely conserved cyclin family, whose customers are characterised by a spectacular periodicity in protein abundance from the mobile cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins show distinctive expression and degradation styles which lead towards the temporal coordination website of each and every mitotic celebration. Cyclin E forms a fancy with and capabilities being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates for the G1-S phase boundary and is degraded as cells progress by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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